Journal: Frontiers in Immunology
Article Title: Developing an in vitro osteochondral micro-physiological system for modeling cartilage-bone crosstalk in arthritis
doi: 10.3389/fimmu.2025.1495613
Figure Lengend Snippet: Inflammatory profile differs slightly in coculture cartilage constructs. (A) Diagram explaining the sample and media collection from the bioreactor coculture. (B) RT-qPCR data showing both a decrease in cartilage anabolic genes and increase in cartilage catabolic genes in inflammatory conditions (n=3 pools, 3 donors/pool, normalized to D0 untreated and HKG RPL13a , Friedman P values: SOX9 = 0.1944, ACAN = 0.1944, COL2A1 = 0.3611, COL1A1 = 0.1944, ADAMTS4 = 0.9444, ADAMTS5 = 0.0278, MMP1 = 0.9444, MMP3 = 0.0278, MMP13 = 0.9444). (C) Pan-MMP assay and MMP1, 3, and 13 ELISA data showing an increase in MMP secretion under inflammatory conditions (n=3 pools, 3 donors/pool, blanked with media and normalized to volume and construct number, Friedman P values: Pan-MMP = 0.3611, MMP1 = 0.0278, MMP3 = 0.0278, MMP13 = 0.3611). (D) Immunofluorescent staining for Collagen Type II showing a decrease in signal under inflammatory conditions (Scale = 50μm).
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were performed according to the manufacturer’s instructions (Peprotech, Cranbury, NJ, ).
Techniques: Construct, Quantitative RT-PCR, Mmp Assay, Enzyme-linked Immunosorbent Assay, Staining