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cytokines il 1β  (Proteintech)


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    Structured Review

    Proteintech cytokines il 1β
    Cytokines Il 1β, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 251 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokines il 1β/product/Proteintech
    Average 96 stars, based on 251 article reviews
    cytokines il 1β - by Bioz Stars, 2026-02
    96/100 stars

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    Inflammatory conditions increase catabolic genes in isolated cartilage constructs. (A) RT-qPCR data showing an increase in catabolic genes in the ADAMTS family and MMP family in inflammatory conditions (n=3 pools, 3 donors/pool, normalized to D0 untreated and HKG RPL13a , Friedman P values: COL1A1 = 0.0278, ADAMTS4 = 0.1944, ADAMTS5 = 0.0278, MMP1 = 0.0278, MMP3 = 0.1944, MMP13 = 0.1944). (B) Pan-MMP assay and MMP1, 3, and 13 <t>ELISA</t> data showing an increase in MMP secretion under inflammatory conditions (n=3 pools, 3 donors/pool, blanked with media and normalized to volume and construct number, Friedman P values: Pan-MMP = 0.1944, MMP1 = 0.1944, MMP3 = 0.1944, MMP13 = 0.1944).
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    Inflammatory conditions increase catabolic genes in isolated cartilage constructs. (A) RT-qPCR data showing an increase in catabolic genes in the ADAMTS family and MMP family in inflammatory conditions (n=3 pools, 3 donors/pool, normalized to D0 untreated and HKG RPL13a , Friedman P values: COL1A1 = 0.0278, ADAMTS4 = 0.1944, ADAMTS5 = 0.0278, MMP1 = 0.0278, MMP3 = 0.1944, MMP13 = 0.1944). (B) Pan-MMP assay and MMP1, 3, and 13 ELISA data showing an increase in MMP secretion under inflammatory conditions (n=3 pools, 3 donors/pool, blanked with media and normalized to volume and construct number, Friedman P values: Pan-MMP = 0.1944, MMP1 = 0.1944, MMP3 = 0.1944, MMP13 = 0.1944).

    Journal: Frontiers in Immunology

    Article Title: Developing an in vitro osteochondral micro-physiological system for modeling cartilage-bone crosstalk in arthritis

    doi: 10.3389/fimmu.2025.1495613

    Figure Lengend Snippet: Inflammatory conditions increase catabolic genes in isolated cartilage constructs. (A) RT-qPCR data showing an increase in catabolic genes in the ADAMTS family and MMP family in inflammatory conditions (n=3 pools, 3 donors/pool, normalized to D0 untreated and HKG RPL13a , Friedman P values: COL1A1 = 0.0278, ADAMTS4 = 0.1944, ADAMTS5 = 0.0278, MMP1 = 0.0278, MMP3 = 0.1944, MMP13 = 0.1944). (B) Pan-MMP assay and MMP1, 3, and 13 ELISA data showing an increase in MMP secretion under inflammatory conditions (n=3 pools, 3 donors/pool, blanked with media and normalized to volume and construct number, Friedman P values: Pan-MMP = 0.1944, MMP1 = 0.1944, MMP3 = 0.1944, MMP13 = 0.1944).

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were performed according to the manufacturer’s instructions (Peprotech, Cranbury, NJ, ).

    Techniques: Isolation, Construct, Quantitative RT-PCR, Mmp Assay, Enzyme-linked Immunosorbent Assay

    Inflammatory profile differs slightly in coculture cartilage constructs. (A) Diagram explaining the sample and media collection from the bioreactor coculture. (B) RT-qPCR data showing both a decrease in cartilage anabolic genes and increase in cartilage catabolic genes in inflammatory conditions (n=3 pools, 3 donors/pool, normalized to D0 untreated and HKG RPL13a , Friedman P values: SOX9 = 0.1944, ACAN = 0.1944, COL2A1 = 0.3611, COL1A1 = 0.1944, ADAMTS4 = 0.9444, ADAMTS5 = 0.0278, MMP1 = 0.9444, MMP3 = 0.0278, MMP13 = 0.9444). (C) Pan-MMP assay and MMP1, 3, and 13 ELISA data showing an increase in MMP secretion under inflammatory conditions (n=3 pools, 3 donors/pool, blanked with media and normalized to volume and construct number, Friedman P values: Pan-MMP = 0.3611, MMP1 = 0.0278, MMP3 = 0.0278, MMP13 = 0.3611). (D) Immunofluorescent staining for Collagen Type II showing a decrease in signal under inflammatory conditions (Scale = 50μm).

    Journal: Frontiers in Immunology

    Article Title: Developing an in vitro osteochondral micro-physiological system for modeling cartilage-bone crosstalk in arthritis

    doi: 10.3389/fimmu.2025.1495613

    Figure Lengend Snippet: Inflammatory profile differs slightly in coculture cartilage constructs. (A) Diagram explaining the sample and media collection from the bioreactor coculture. (B) RT-qPCR data showing both a decrease in cartilage anabolic genes and increase in cartilage catabolic genes in inflammatory conditions (n=3 pools, 3 donors/pool, normalized to D0 untreated and HKG RPL13a , Friedman P values: SOX9 = 0.1944, ACAN = 0.1944, COL2A1 = 0.3611, COL1A1 = 0.1944, ADAMTS4 = 0.9444, ADAMTS5 = 0.0278, MMP1 = 0.9444, MMP3 = 0.0278, MMP13 = 0.9444). (C) Pan-MMP assay and MMP1, 3, and 13 ELISA data showing an increase in MMP secretion under inflammatory conditions (n=3 pools, 3 donors/pool, blanked with media and normalized to volume and construct number, Friedman P values: Pan-MMP = 0.3611, MMP1 = 0.0278, MMP3 = 0.0278, MMP13 = 0.3611). (D) Immunofluorescent staining for Collagen Type II showing a decrease in signal under inflammatory conditions (Scale = 50μm).

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were performed according to the manufacturer’s instructions (Peprotech, Cranbury, NJ, ).

    Techniques: Construct, Quantitative RT-PCR, Mmp Assay, Enzyme-linked Immunosorbent Assay, Staining

    Inflammatory profile of vascularized-bone construct shows evidence of cartilage-bone crosstalk. (A) Diagram explaining the sample and media collection from the bioreactor coculture. (B) RT-qPCR data showing both a decrease in most bone anabolic genes and increase in bone catabolic genes in inflammatory conditions (n=3 pools, 3 donors/pool, normalized to D0 untreated and HKG RPL13a , Friedman P values: IBSP = 0.1944, RUNX2 = 0.9444, ALPL = 0.1944, SPP1 = 0.1944, BGLAP = 0.1944, COL1A1 = 0.1944, COL10A1 > 0.9999, MMP1 = 0.1944, MMP3 = 0.1944, MMP13 = 0.1944). (C) Pan-MMP assay and MMP1, 3, and 13 ELISA data showing an increase in MMP secretion under inflammatory conditions (n=3 pools, 3 donors/pool, blanked with media and normalized to volume and construct number, Friedman P values: Pan-MMP = 0.1944, MMP1 = 0.5278, MMP3 = 0.0278, MMP13 = 0.1944).

    Journal: Frontiers in Immunology

    Article Title: Developing an in vitro osteochondral micro-physiological system for modeling cartilage-bone crosstalk in arthritis

    doi: 10.3389/fimmu.2025.1495613

    Figure Lengend Snippet: Inflammatory profile of vascularized-bone construct shows evidence of cartilage-bone crosstalk. (A) Diagram explaining the sample and media collection from the bioreactor coculture. (B) RT-qPCR data showing both a decrease in most bone anabolic genes and increase in bone catabolic genes in inflammatory conditions (n=3 pools, 3 donors/pool, normalized to D0 untreated and HKG RPL13a , Friedman P values: IBSP = 0.1944, RUNX2 = 0.9444, ALPL = 0.1944, SPP1 = 0.1944, BGLAP = 0.1944, COL1A1 = 0.1944, COL10A1 > 0.9999, MMP1 = 0.1944, MMP3 = 0.1944, MMP13 = 0.1944). (C) Pan-MMP assay and MMP1, 3, and 13 ELISA data showing an increase in MMP secretion under inflammatory conditions (n=3 pools, 3 donors/pool, blanked with media and normalized to volume and construct number, Friedman P values: Pan-MMP = 0.1944, MMP1 = 0.5278, MMP3 = 0.0278, MMP13 = 0.1944).

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were performed according to the manufacturer’s instructions (Peprotech, Cranbury, NJ, ).

    Techniques: Construct, Quantitative RT-PCR, Mmp Assay, Enzyme-linked Immunosorbent Assay